Undercover security guards will be deployed in the Twickenham crowd for Saturday's Premiership final between Northampton and Bath.
Last year's showpiece fixture between Saracens and Sale was disrupted by Just Stop Oil protestors, with flanker Tom Curry having to drag one of them off the pitch.
The match was just one of a number of sporting events briefly suspended by the climate activist group in 2023, who also protested at Wimbledon, the snooker World Championship at the Crucible, and Lord's during last summer's Ashes.
There are also heightened security concerns after three people invaded the pitch at Wembley during last weekend's Champions League final between Real Madrid and Dortmund.
Mail Sport's Aadam Patel went undercover during the European clash to expose the ease at which ticketless fans could gain access to the home of English football, spending just £150 to pay off a steward.
Twickenham will deploy undercover security forces for Saturday's Premiership final between Bath and Northampton
Last year's final was infiltrated by climate activists from Just Stop Oil who made it to the pitch
Premiership Rugby's chief growth officer called the fracas at last year's event 'regrettable'
This weekend's match is an 82,000 sell-out and an RFU spokesperson said: 'Our security arrangements are continually assessed and proportionate to the size and scale of event taking place at the stadium at any given time.'
Rob Calder, Premiership Rugby's chief growth officer, added: 'Last year was regrettable.
'The RFU are clearly responsible for anything that happens of that nature so they are all over it - fingers crossed there won't be anything like that again.'